| Unlockable: | How to Unlock: |
| Ace | Complete the Story Mode with two different characters. |
| Blackbeard | Complete the Story Mode with Shanks. |
| Crocodile | Complete the Story Mode with three different characters. |
| Ener | Complete the Story Mode with five different characters. |
| Mihawk | Complete the Story Mode with all other characters. |
| Shanks | Complete the Story Mode with Mihawk. |
| Smoker | Complete the Story Mode with one character. |
| Vivi | Complete the Story Mode with four different characters. |
